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rnase h  (New England Biolabs)


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    Structured Review

    New England Biolabs rnase h
    Rnase H, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnase+a/RNase+H/pmc12926610-373-32-34
    Average 99 stars, based on 5330 article reviews
    rnase h - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Genome assemblies of the daisy Gorteria diffusa elucidate the molecular-genomic basis of pollination by sexual deception.
    Article Snippet: .. Briefly, 500 μl of CTAB buffer was 14 added to approximately 20 mg of leaf powder, vortexed, and incubated at 55°C for 1 h. 15 Subsequently, 5 μl of RNase A (New England Biolabs, USA) was added, and the samples 16 were incubated for 30 minutes at 37°C. ..

    Article Title: ZAP targets aberrant mRNA transcripts encoding proteins with defective signal peptides for degradation.
    Article Snippet: In total, 50 million K562 ZAP-S KO cells stably expressing HAtagged ZAP-S were harvested and lysed in lysis buffer (50 mM HEPES [pH 8], 150 mM NaCl, 5 mM MgCl2, 1% Triton X-100) supplemented with 2U Turbo DNase (Thermo Fisher Scientific, AM2238) as well as protease (Sigma, 11836170001) and phosphatase (Sigma, PHOSS-RO) inhibitor cocktail. .. In all, 10 μg/mL RNase A (New England Biolabs, T3018) was added to the clarified lysate, followed by 40U murine RNase inhibitor (New England Biolabs, M0314L) to inactive the RNase A. ZAP-S complex was then immunoprecipitated by incubation with pre-equilibrated anti-HA magnetic beads (Thermo Fisher Scientific, 88836) for 1 h at room temperature. ..

    Article Title: ZAP targets aberrant mRNA transcripts encoding proteins with defective signal peptides for degradation
    Article Snippet: In total, 50 million K562 ZAP-S KO cells stably expressing HA-tagged ZAP-S were harvested and lysed in lysis buffer (50 mM HEPES [pH 8], 150 mM NaCl, 5 mM MgCl 2 , 1% Triton X-100) supplemented with 2U Turbo DNase (Thermo Fisher Scientific, AM2238) as well as protease (Sigma, 11836170001) and phosphatase (Sigma, PHOSS-RO) inhibitor cocktail. .. In all, 10 μg/mL RNase A (New England Biolabs, T3018) was added to the clarified lysate, followed by 40U murine RNase inhibitor (New England Biolabs, M0314L) to inactive the RNase A. ZAP-S complex was then immunoprecipitated by incubation with pre-equilibrated anti-HA magnetic beads (Thermo Fisher Scientific, 88836) for 1 h at room temperature. ..

    Article Title: A Dimeric Rocaglate Promotes Multivalent eIF4A-RNA Assembly
    Article Snippet: Normalized lysates (400 μg) were incubated overnight at 4 °C with 2.5 μg anti-eIF4A1 (Abcam, ab31217), anti-eIF4A2 (Santa Cruz Biotechnology, SC-137148), or IgG control antibodies (Cell Signaling Technology, 3900S or 5415S). .. Where indicated, RNase A (10 μg/mL; NEB, T3018L) was added during the incubation. .. Protein A/G magnetic beads (25 μL bead slurry; Thermo Fisher, 88803) were equilibrated, added to the samples, and incubated at room temperature for 1 h. Beads were collected on a magnetic rack and washed twice with IP lysis buffer and once with water.

    Article Title: Altered chromatin accessibility and nucleosome positioning landscape upon HDAC and LSD1 inhibition in cancer cell
    Article Snippet: .. 3 μl of RNAse A (NEB # T3018L) was also added to the labeling reaction and incubated at 37 °C for 30 min to digest cellular RNA. .. Slides were washed once with NEED-seq washing buffer (20 mM phosphate buffer, 0.5 M NaCl, and 1 % Triton X-100) and were air dried, mounted using Prolong Gold antifade reagent with DAPI (Invitrogen, P36935) and visualized using LSM 880 confocal microscope (Zeiss).

    Immunoprecipitation:

    Article Title: ZAP targets aberrant mRNA transcripts encoding proteins with defective signal peptides for degradation.
    Article Snippet: In total, 50 million K562 ZAP-S KO cells stably expressing HAtagged ZAP-S were harvested and lysed in lysis buffer (50 mM HEPES [pH 8], 150 mM NaCl, 5 mM MgCl2, 1% Triton X-100) supplemented with 2U Turbo DNase (Thermo Fisher Scientific, AM2238) as well as protease (Sigma, 11836170001) and phosphatase (Sigma, PHOSS-RO) inhibitor cocktail. .. In all, 10 μg/mL RNase A (New England Biolabs, T3018) was added to the clarified lysate, followed by 40U murine RNase inhibitor (New England Biolabs, M0314L) to inactive the RNase A. ZAP-S complex was then immunoprecipitated by incubation with pre-equilibrated anti-HA magnetic beads (Thermo Fisher Scientific, 88836) for 1 h at room temperature. ..

    Article Title: ZAP targets aberrant mRNA transcripts encoding proteins with defective signal peptides for degradation
    Article Snippet: In total, 50 million K562 ZAP-S KO cells stably expressing HA-tagged ZAP-S were harvested and lysed in lysis buffer (50 mM HEPES [pH 8], 150 mM NaCl, 5 mM MgCl 2 , 1% Triton X-100) supplemented with 2U Turbo DNase (Thermo Fisher Scientific, AM2238) as well as protease (Sigma, 11836170001) and phosphatase (Sigma, PHOSS-RO) inhibitor cocktail. .. In all, 10 μg/mL RNase A (New England Biolabs, T3018) was added to the clarified lysate, followed by 40U murine RNase inhibitor (New England Biolabs, M0314L) to inactive the RNase A. ZAP-S complex was then immunoprecipitated by incubation with pre-equilibrated anti-HA magnetic beads (Thermo Fisher Scientific, 88836) for 1 h at room temperature. ..

    Magnetic Beads:

    Article Title: ZAP targets aberrant mRNA transcripts encoding proteins with defective signal peptides for degradation.
    Article Snippet: In total, 50 million K562 ZAP-S KO cells stably expressing HAtagged ZAP-S were harvested and lysed in lysis buffer (50 mM HEPES [pH 8], 150 mM NaCl, 5 mM MgCl2, 1% Triton X-100) supplemented with 2U Turbo DNase (Thermo Fisher Scientific, AM2238) as well as protease (Sigma, 11836170001) and phosphatase (Sigma, PHOSS-RO) inhibitor cocktail. .. In all, 10 μg/mL RNase A (New England Biolabs, T3018) was added to the clarified lysate, followed by 40U murine RNase inhibitor (New England Biolabs, M0314L) to inactive the RNase A. ZAP-S complex was then immunoprecipitated by incubation with pre-equilibrated anti-HA magnetic beads (Thermo Fisher Scientific, 88836) for 1 h at room temperature. ..

    Article Title: ZAP targets aberrant mRNA transcripts encoding proteins with defective signal peptides for degradation
    Article Snippet: In total, 50 million K562 ZAP-S KO cells stably expressing HA-tagged ZAP-S were harvested and lysed in lysis buffer (50 mM HEPES [pH 8], 150 mM NaCl, 5 mM MgCl 2 , 1% Triton X-100) supplemented with 2U Turbo DNase (Thermo Fisher Scientific, AM2238) as well as protease (Sigma, 11836170001) and phosphatase (Sigma, PHOSS-RO) inhibitor cocktail. .. In all, 10 μg/mL RNase A (New England Biolabs, T3018) was added to the clarified lysate, followed by 40U murine RNase inhibitor (New England Biolabs, M0314L) to inactive the RNase A. ZAP-S complex was then immunoprecipitated by incubation with pre-equilibrated anti-HA magnetic beads (Thermo Fisher Scientific, 88836) for 1 h at room temperature. ..

    Labeling:

    Article Title: Altered chromatin accessibility and nucleosome positioning landscape upon HDAC and LSD1 inhibition in cancer cell
    Article Snippet: .. 3 μl of RNAse A (NEB # T3018L) was also added to the labeling reaction and incubated at 37 °C for 30 min to digest cellular RNA. .. Slides were washed once with NEED-seq washing buffer (20 mM phosphate buffer, 0.5 M NaCl, and 1 % Triton X-100) and were air dried, mounted using Prolong Gold antifade reagent with DAPI (Invitrogen, P36935) and visualized using LSM 880 confocal microscope (Zeiss).



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